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验证数据展示

  • IF Staining of HepG2 using CL488-84854-5

    IF Staining of HepG2 using CL488-84854-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CD44 antibody (CL488-84854-5, Clone: 242345G11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CD44 Recombinant antibody

CD44 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84854-5

产品说明书

CloneNo. 242345G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

242345G11, CD44 antigen, CDW44, CSPG8, ECMR III

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84854-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84854-5 targets CD44 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CD44 fusion protein Eg1057 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD44 molecule (Indian blood group)
别名 242345G11, CD44 antigen, CDW44, CSPG8, ECMR III
计算分子量82 kDa
观测分子量80-95 kDa
GenBank蛋白编号NM_000610.3
基因名称 CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16070-1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD44 antibody CL488-84854-5Download protocol
Standard Protocols
Click here to view our Standard Protocols