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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84854-5-RR (same clone as 84854-5-PBS)

    WB analysis using 84854-5-RR (same clone as 84854-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84854-5-RR (CD44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

    IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

    IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • IF Staining of A431 using 84854-5-RR (same clone as 84854-5-PBS)

    IF Staining of A431 using 84854-5-RR (same clone as 84854-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (84854-5-RR, Clone: 242345G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84854-5-RR

    Affinity and Kinetic Characterization of 84854-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84854-5-RR against Human CD44 were performed. The affinity constant is 1.01 nM.

CD44 Recombinant antibody, PBS Only

CD44 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84854-5-PBS

产品说明书

CloneNo. 242345G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

产品信息

84854-5-PBS targets CD44 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD44 molecule (Indian blood group)
别名 ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen
计算分子量82 kDa
观测分子量80-95 kDa
GenBank蛋白编号NM_000610.3
基因名称 CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16070-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.