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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

    FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

    FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98522-3-RR

    Affinity and Kinetic Characterization of 98522-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98522-3-RR against Mouse CD4 were performed. The affinity constant is below 1 pM.

Anti-Mouse CD4 Rabbit Recombinant Antibody, PBS Only

CD4 Uni-rAb® Recombinant Antibody for FC
Cat No. 98522-3-PBS

产品说明书

CloneNo. 250263B7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98522-3-PBS targets CD4 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3847

Product name: Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 27-394 aa of NM_013488

Sequence: KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD4 antigen
别名 CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
计算分子量51 kDa
GenBank蛋白编号NM_013488
基因名称 Cd4
Gene ID (NCBI) 12504
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06332
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).