验证数据展示
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65077-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | γδTCR-positive T-T hybridoma D1 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD3 antigen, epsilon polypeptide |
| 别名 | AI504783, CD3, Cd3e, CD3epsilon, T3e |
| GenBank蛋白编号 | BC098236 |
| 基因名称 | CD3ε |
| Gene ID (NCBI) | 12501 |
| RRID | AB_2883666 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P22646 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CD3 antibody CL647-65077 | Download protocol |
| FC protocol for CL Plus 647 CD3 antibody CL647-65077 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |