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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 98601-2-RR (same clone as 98601-2-PBS)

    FC experiment of human peripheral blood lymphocytes using 98601-2-RR (same clone as 98601-2-PBS)

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD32 Rabbit RecAb (98601-2-RR, Clone: 251087D9) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human HLA-DR. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98601-2-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood lymphocytes using 98601-2-RR (same clone as 98601-2-PBS)

    FC experiment of human peripheral blood lymphocytes using 98601-2-RR (same clone as 98601-2-PBS)

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD32 Rabbit RecAb (98601-2-RR, Clone: 251087D9) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98601-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98601-2-RR

    Affinity and Kinetic Characterization of 98601-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98601-2-RR against Human CD32 were performed. The affinity constant is 4.86 nM.

Anti-Human CD32 Rabbit Recombinant Antibody, PBS Only

CD32 Uni-rAb® Recombinant Antibody for FC
Cat No. 98601-2-PBS

产品说明书

CloneNo. 251087D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

产品信息

98601-2-PBS targets CD32 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2713

Product name: Recombinant Human FCGR2A/CD32a protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 34-217 aa of NM_001136219.3

Sequence: QAAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a
计算分子量35 kDa
GenBank蛋白编号NM_001136219.3
基因名称 CD32a
Gene ID (NCBI) 2212
RRIDAB_3746209
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12318-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

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