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验证数据展示

  • IF Staining of mouse brain using CL555-28083

    IF Staining of mouse brain using CL555-28083

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 555 CD31 antibody (CL555-28083) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 555-conjugated CD31 Polyclonal antibody

CD31 Polyclonal Antibody for IF-P
Cat No. CL555-28083

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF-P

别名

Pecam, PECAM 1, Pecam1, PECAM-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 28083-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-28083 targets CD31 in IF-P applications and shows reactivity with mouse samples.

经测试应用 IF-P Application Description
经测试反应性 mouse
免疫原 CD31 fusion protein Ag27828 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule 1
别名 Pecam, PECAM 1, Pecam1, PECAM-1
计算分子量 81 kDa
观测分子量 110-130 kDa
GenBank蛋白编号NM_001032378
基因名称 CD31
Gene ID (NCBI) 18613
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08481
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 CD31 antibody CL555-28083Download protocol
Standard Protocols
Click here to view our Standard Protocols