验证数据展示

WB analysis of bEnd.3 using 28083-1-AP
bEnd.3 cells were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 28083-1-AP

产品说明书

引用文献(121)

宿主/亚型

Rabbit / IgG

种属反应性

mouse and More (1)

应用

WB, IHC, IF-P, IF-Fro, ELISA and More (1)

别名

Pecam, PECAM 1, Pecam1, PECAM-1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (121)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	bEnd.3 cells, mouse liver tissue, mouse spleen tissue
Positive IHC detected in	mouse liver tissue, mouse brain tissue, mouse kidney tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue, mouse kidney tissue
Positive IF-Fro detected in	mouse brain tissue

Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 17 publications below
IHC	See 46 publications below
IF	See 60 publications below

产品信息

28083-1-AP targets CD31 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse samples.

经测试应用	WB, IHC, IF-P, IF-Fro, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	mouse
文献引用反应性	human, mouse
免疫原	CatNo: Ag27828 Product name: Recombinant mouse Pecam1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 51-276 aa of NM_001032378 Sequence: MISTTSKSRSQHRVLFYKDDAMVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTVNCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEPIRSEYVTVQESFSTPKFEIKPPGMIIEGDQLHIRCIVQVTHLVQEFTEIIIQKDKAIVATSKQSSE 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	platelet/endothelial cell adhesion molecule 1
别名	Pecam, PECAM 1, Pecam1, PECAM-1
计算分子量	81 kDa
观测分子量	110-130 kDa
GenBank蛋白编号	NM_001032378
基因名称	CD31
Gene ID (NCBI)	18613
RRID	AB_2881055
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q08481
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CD31 antibody 28083-1-AP	Download protocol
IHC protocol for CD31 antibody 28083-1-AP	Download protocol
WB protocol for CD31 antibody 28083-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	IHC	Nanomicro Lett Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring Authors - Yuewei Chen View Article
mouse		Cell Mol Biol Lett Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions Authors - Seyram Yao Adzraku View Article
mouse	IHC	Front Immunol Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis Authors - Fengxia Bai View Article
mouse	IF	Cells Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars Authors - Jorge Luis Montenegro Raudales View Article
mouse	IF	J Ethnopharmacol The mechanism of Chebulae Fructus Immaturus promote diabetic wound healing based on network pharmacology and experimental verification Authors - Feng Qiu View Article
mouse	IF	Front Immunol "γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy. Authors - Zhigang Zhang View Article

验证数据展示

WB analysis of bEnd.3 using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

IHC staining of mouse liver using 28083-1-AP

IHC staining of mouse liver using 28083-1-AP

IHC staining of mouse kidney using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions

Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis

Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars

The mechanism of Chebulae Fructus Immaturus promote diabetic wound healing based on network pharmacology and experimental verification

"γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy.