×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 66065-2-Ig

    WB analysis using 66065-2-Ig

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IF Staining of HUVEC using 66065-2-Ig

    IF Staining of HUVEC using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of human peripheral blood platelets using 66065-2-Ig

    WB analysis of human peripheral blood platelets using 66065-2-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • WB analysis using 66065-2-Ig

    WB analysis using 66065-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 66065-2-Ig

    IHC staining of human stomach cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 66065-2-Ig

    IHC staining of human oesophagus cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 66065-2-Ig

    IHC staining of human placenta using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66065-2-Ig

    IHC staining of human pancreas cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 66065-2-Ig

    IHC staining of human appendicitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 66065-2-Ig

    IHC staining of human pancreas using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human pancreas using 66065-2-Ig

    IHC staining of human pancreas using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human appendicitis using 66065-2-Ig

    IHC staining of human appendicitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human breast cancer using 66065-2-Ig

    IHC staining of human breast cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human colon cancer using 66065-2-Ig

    IHC staining of human colon cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human pancreas cancer using 66065-2-Ig

    IHC staining of human pancreas cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human placenta using 66065-2-Ig

    IHC staining of human placenta using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human oesophagus cancer using 66065-2-Ig

    IHC staining of human oesophagus cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IF Staining of human placenta using 66065-2-Ig

    IF Staining of human placenta using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 66065-2-Ig

    IF Staining of human placenta using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 66065-2-Ig

    IF Staining of HUVEC using 66065-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).

CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66065-2-Ig

产品说明书

CloneNo. 3F8E2
引用文献(126)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

3F8E2, EndoCAM, GPIIA, GPIIA', PECA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue, human liver cancer tissue
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66065-2-Ig targets CD31 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, pig, rabbit
免疫原

CatNo: Ag19730

Product name: Recombinant human CD31 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 428-738 aa of BC022512

Sequence: EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 3F8E2, EndoCAM, GPIIA, GPIIA', PECA1
计算分子量 83 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2918476
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CD31 antibody 66065-2-IgDownload protocol
IHC protocol for CD31 antibody 66065-2-IgDownload protocol
WB protocol for CD31 antibody 66065-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

ACS Nano

Collagen-Targeting Self-Assembled Nanoprobes for Multimodal Molecular Imaging and Quantification of Myocardial Fibrosis in a Rat Model of Myocardial Infarction

Authors - Fang Li
View Article
humanIHC

J Exp Clin Cancer Res

CD151-enriched migrasomes mediate hepatocellular carcinoma invasion by conditioning cancer cells and promoting angiogenesis

Authors - Kangnan Zhang
View Article
IF

Cardiovasc Diabetol

Deficiency of neutral cholesterol ester hydrolase 1 (NCEH1) impairs endothelial function in diet-induced diabetic mice

Authors - Hai-Jian Sun
View Article
WB,IF

J Neuroinflammation

Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

Authors - Tianyi Shen
View Article
IF

JCI Insight

Uric acid formation is driven by crosstalk between skeletal muscle and other cell types

Authors - Spencer G Miller
View Article
IF

Phytomedicine

Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis

Authors - Zixian Zhou
View Article
||
New chat

Able™

正在加载，请稍候...