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验证数据展示

  • WB analysis using 66065-2-Ig

    WB analysis using 66065-2-Ig

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IF Staining of HUVEC using 66065-2-Ig

    IF Staining of HUVEC using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of human peripheral blood platelets using 66065-2-Ig

    WB analysis of human peripheral blood platelets using 66065-2-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • WB analysis using 66065-2-Ig

    WB analysis using 66065-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 66065-2-Ig

    IHC staining of human stomach cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human oesophagus cancer using 66065-2-Ig

    IHC staining of human oesophagus cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human placenta using 66065-2-Ig

    IHC staining of human placenta using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 66065-2-Ig

    IHC staining of human pancreas cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66065-2-Ig

    IHC staining of human appendicitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas using 66065-2-Ig

    IHC staining of human pancreas using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human pancreas using 66065-2-Ig

    IHC staining of human pancreas using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human appendicitis using 66065-2-Ig

    IHC staining of human appendicitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human breast cancer using 66065-2-Ig

    IHC staining of human breast cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human colon cancer using 66065-2-Ig

    IHC staining of human colon cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver using 66065-2-Ig

    IHC staining of human liver using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig

    IHC staining of human liver cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human pancreas cancer using 66065-2-Ig

    IHC staining of human pancreas cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human tonsillitis using 66065-2-Ig

    IHC staining of human tonsillitis using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human placenta using 66065-2-Ig

    IHC staining of human placenta using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human oesophagus cancer using 66065-2-Ig

    IHC staining of human oesophagus cancer using 66065-2-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD31 antibody included
  • IF Staining of human placenta using 66065-2-Ig

    IF Staining of human placenta using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 66065-2-Ig

    IF Staining of human placenta using 66065-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 66065-2-Ig

    IF Staining of HUVEC using 66065-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).

CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66065-2-Ig

产品说明书

CloneNo. 3F8E2
引用文献(137)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

3F8E2, EndoCAM, GPIIA, GPIIA', PECA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman peripheral blood platelets, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human oesophagus cancer tissue, human liver tissue, human tonsillitis tissue, human pancreas tissue, human placenta tissue, human pancreas cancer tissue, human colon cancer tissue, human liver cancer tissue, human appendicitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue, human liver cancer tissue
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66065-2-Ig targets CD31 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, pig, rabbit
免疫原

CatNo: Ag19730

Product name: Recombinant human CD31 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 428-738 aa of BC022512

Sequence: EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 3F8E2, EndoCAM, GPIIA, GPIIA', PECA1
计算分子量 83 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2918476
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CD31 antibody 66065-2-IgDownload protocol
IHC protocol for CD31 antibody 66065-2-IgDownload protocol
WB protocol for CD31 antibody 66065-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Cell

Conserved spatial subtypes and cellular neighborhoods of cancer-associated fibroblasts revealed by single-cell spatial multi-omics

Authors - Yunhe Liu
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humanWB

Cell

ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization

Authors - Yuqi Wang
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humanIF

Nat Nanotechnol

Prevention of acute thrombosis with vascular endothelium antioxidative nanoscavenger.

Authors - Yixin Zhong
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IF

Cancer Commun (Lond)

Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis.

Authors - Yizhi Zhan
View Article
IF

Bioact Mater

Throw out an oligopeptide to catch a protein: Deep learning and natural language processing-screened tripeptide PSP promotes Osteolectin-mediated vascularized bone regeneration

Authors - Yu Chen
View Article
IHC

Nat Commun

Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer

Authors - Xu Guan
View Article
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