验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 98160-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-98160 targets CD27 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD27 antigen |
| 别名 | Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen |
| 计算分子量 | 28kDa |
| GenBank蛋白编号 | NM_001033126.2 |
| 基因名称 | CD27 |
| Gene ID (NCBI) | 21940 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P41272 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD27 antibody CL750-98160 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
