• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated CD27 Monoclonal antibody

CD27 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C1G3

Cat No : CL594-66308

Print datasheet

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

验证数据展示

  • IF Staining of human tonsillitis using CL594-66308

    IF Staining of human tonsillitis using CL594-66308

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66308 (CD27 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66308-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66308 targets CD27 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen CD27 fusion protein Ag24537 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD27 molecule
Synonyms CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2883553
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26842
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
IF protocol for CL594 CD27 antibody CL594-66308Download protocol
Standard Protocols
Click here to view our Standard Protocols