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CD27 Monoclonal antibody

CD27 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1G3

Cat No : 66308-1-Ig

Print datasheet

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

验证数据展示

  • WB analysis using 66308-1-Ig

    WB analysis using 66308-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66308-1-Ig

    IHC staining of human tonsillitis using 66308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66308-1-Ig

    IHC staining of human tonsillitis using 66308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 66308-1-Ig

    IF Staining of human tonsillitis using 66308-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 66308-1-Ig

    IF Staining of human tonsillitis using 66308-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Raji using 66308-1-Ig

    FC experiment of Raji using 66308-1-Ig

    1X10^6 Raji cells were surface stained with 0.2 ug Anti-Human CD27 (66308-1-Ig, Clone:1C1G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were not fixed.

经过测试的应用

Positive WB detected inRaji cells, pig spleen tissue, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRaji cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66308-1-Ig targets CD27 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, pig
Immunogen CD27 fusion protein Ag24537 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD27 molecule
Synonyms CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2881690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26842
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
WB protocol for CD27 antibody 66308-1-IgDownload protocol
IHC protocol for CD27 antibody 66308-1-IgDownload protocol
IF protocol for CD27 antibody 66308-1-IgDownload protocol
FC protocol for CD27 antibody 66308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols