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验证数据展示

  • WB analysis using 85066-5-RR (same clone as 85066-5-PBS)

    WB analysis using 85066-5-RR (same clone as 85066-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85066-5-RR (CD27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    IHC staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85066-5-RR (CD27 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    IHC staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85066-5-RR (CD27 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    IF Staining of human tonsillitis using 85066-5-RR (same clone as 85066-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

  • IF Staining of Ramos using 85066-5-RR (same clone as 85066-5-PBS)

    IF Staining of Ramos using 85066-5-RR (same clone as 85066-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85066-5-PBS in a different storage buffer formulation.

CD27 Recombinant monoclonal antibody, PBS Only

CD27 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 85066-5-PBS

产品说明书

CloneNo. 242731F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

242731F2, CD27 antigen, CD27 molecule, CD27L receptor, S152

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85066-5-PBS targets CD27 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1644

Product name: Recombinant Human CD27 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-191 aa of BC012160

Sequence: ATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD27 molecule
别名 242731F2, CD27 antigen, CD27 molecule, CD27L receptor, S152
计算分子量 29 kDa
观测分子量50 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26842
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

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