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验证数据展示

  • WB analysis using 85066-5-RR

    WB analysis using 85066-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85066-5-RR (CD27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 85066-5-RR

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 85066-5-RR

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Ramos using 85066-5-RR

    IF Staining of Ramos using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD27 Recombinant antibody

CD27 Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 85066-5-RR

产品说明书

CloneNo. 242731F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IF-P, ELISA

别名

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRamos cells
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inRamos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85066-5-RR targets CD27 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD27 molecule
别名 T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen
计算分子量 29 kDa
观测分子量50 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26842
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
WB protocol for CD27 antibody 85066-5-RRDownload protocol
IF protocol for CD27 antibody 85066-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols