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验证数据展示

  • WB analysis using 85066-5-RR

    WB analysis using 85066-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85066-5-RR (CD27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 85066-5-RR

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 85066-5-RR

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Ramos using 85066-5-RR

    IF Staining of Ramos using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD27 Recombinant antibody

CD27 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 85066-5-RR

产品说明书

CloneNo. 242731F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IF-P, ELISA

别名

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRamos cells
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inRamos cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85066-5-RR targets CD27 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD27 molecule
别名 T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen
计算分子量 29 kDa
观测分子量50 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26842
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
WB protocol for CD27 antibody 85066-5-RRDownload protocol
IF protocol for CD27 antibody 85066-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols