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PE Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2b

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HIR2

Cat No : PE-65174

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • FC experiment of human red blood cells using PE-65174

    FC experiment of human red blood cells using PE-65174

    Human red blood cells were surface stained with PE-Anti-Human CD235a (PE-65174, Clone:HIR2) (red) or PE-Mouse IgG2b Isotype Control (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman red blood cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65174 targets CD235a in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen Synthetic peptide (Human, N-terminal) 种属同源性预测
Host / Isotype Mouse / Mouse IgG2b
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
GenBank Accession NumberBC005319
Gene Symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2883948
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP02724
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for PE CD235a antibody PE-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols