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验证数据展示

  • FC experiment of human whole blood using CL488-65174

    FC experiment of human whole blood using CL488-65174

    10 ul human whole blood was surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD235a (HIR2) (CL488-65174) or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

  • FC experiment of human whole blood using CL488-65174

    FC experiment of human whole blood using CL488-65174

    10 ul human whole blood was surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD235a (HIR2) (CL488-65174) or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

CoraLite® Plus 488 Anti-Human Glycophorin A/CD235a (HIR2)

Glycophorin A/CD235a Monoclonal Antibody for FC
Cat No. CL488-65174

产品说明书

CloneNo. HIR2

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

human

应用

FC

别名

Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman whole blood
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65174-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10 ul cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65174 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik
计算分子量 150 aa, 16 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_3672869
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Affinity purification
UNIPROT IDP02724
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Glycophorin A/CD235a antibody CL488-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols