验证数据展示
经过测试的应用
| Positive FC detected in | Anti-CD3/CD28 treated mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65098-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
Murine CD223-Ig fusion protein 种属同源性预测 |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte-activation gene 3 |
| 别名 | CD223, LAG-3, Ly66, Lag3, LAG 3 |
| GenBank蛋白编号 | BC120591 |
| 基因名称 | CD223 |
| Gene ID (NCBI) | 16768 |
| RRID | AB_2919262 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q61790 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 LAG-3/CD223 antibody CL488-65098 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
