验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-31909 targets CD20 in IF-P applications and shows reactivity with mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag36918 Product name: Recombinant mouse Ms4a1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-291 aa of NM_007641 Sequence: GIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | membrane-spanning 4-domains, subfamily A, member 1 |
| 别名 | B-cell differentiation antigen Ly-44, B-lymphocyte antigen CD20, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1 |
| 计算分子量 | 32 kDa |
| GenBank蛋白编号 | NM_007641 |
| 基因名称 | Ms4a1 |
| Gene ID (NCBI) | 12482 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P19437 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PE CD20 antibody PE-31909 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
