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验证数据展示

  • WB analysis using 31909-1-AP

    WB analysis using 31909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31909-1-AP (CD20 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31909-1-AP

    IHC staining of mouse spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 31909-1-AP

    IHC staining of rat spleen using 31909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 31909-1-AP

    IF Staining of mouse colon using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 31909-1-AP

    IF Staining of mouse spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat spleen using 31909-1-AP

    IF Staining of rat spleen using 31909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD20 Polyclonal antibody

CD20 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31909-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Membrane-spanning 4-domains subfamily A member 1, Lymphocyte antigen 44, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse colon tissue, rat spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31909-1-AP targets CD20 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 CD20 fusion protein Ag36918 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 Membrane-spanning 4-domains subfamily A member 1, Lymphocyte antigen 44, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44
计算分子量32 kDa
观测分子量35 kDa
GenBank蛋白编号NM_007641
基因名称 Ms4a1
Gene ID (NCBI) 12482
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP19437
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

实验方案

Product Specific Protocols
WB protocol for CD20 antibody 31909-1-APDownload protocol
IHC protocol for CD20 antibody 31909-1-APDownload protocol
IF protocol for CD20 antibody 31909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols