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验证数据展示

  • WB analysis using 31909-1-AP (same clone as 31909-1-PBS)

    WB analysis using 31909-1-AP (same clone as 31909-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31909-1-AP (CD20 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

    IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

    IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

    IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

    IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

  • IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

    IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

Mouse CD20 Polyclonal antibody, PBS Only

CD20 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31909-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

B-cell differentiation antigen Ly-44, B-lymphocyte antigen CD20, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31909-1-PBS targets CD20 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag36918

Product name: Recombinant mouse Ms4a1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-291 aa of NM_007641

Sequence: GIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 B-cell differentiation antigen Ly-44, B-lymphocyte antigen CD20, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1
计算分子量32 kDa
观测分子量35 kDa
GenBank蛋白编号NM_007641
基因名称 Ms4a1
Gene ID (NCBI) 12482
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP19437
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

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