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CoraLite® Plus 750-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4A7G3

Cat No : CL750-60271

Print datasheet

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

验证数据展示

  • FC experiment of Daudi using CL750-60271

    FC experiment of Daudi using CL750-60271

    1X10^6 Daudi cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human CD20 (CL750-60271, Clone: 4A7G3) (red), or 0.8 ug CoraLite® Plus 750 Mouse IgG2b Isotype Control (MPC-11) (CL750-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inDaudi cells
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-60271 targets CD20 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen CD20 fusion protein Ag17309 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 1
Synonyms B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002807
Gene Symbol MS4A1
Gene ID (NCBI) 931
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11836
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 CD20 antibody CL750-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols