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验证数据展示

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

  • IF Staining of human lung cancer using CL647-60271

    IF Staining of human lung cancer using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 pan-keratin antibody (CL488-26411, green).

  • IF Staining of human tonsillitis using CL647-60271

    IF Staining of human tonsillitis using CL647-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

CoraLite® Plus 647-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for IF-P
Cat No. CL647-60271

产品说明书

CloneNo. 4A7G3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF-P

别名

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman lung cancer tissue, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60271 targets CD20 in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag17309

Product name: Recombinant human MS4A1,CD20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 141-297 aa of BC002807

Sequence: IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
计算分子量 297 aa, 33 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
RRIDAB_2883651
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11836
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD20 antibody CL647-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols