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Conjugate
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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL488-65085

    FC experiment of human peripheral blood lymphocytes using CL488-65085

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD20 (CL488-65085, Clone: 2H7) (red) or isotype control antibody (blue). Cells were not fixed.

CoraLite® Plus 488 Anti-Human CD20 (2H7)

CD20 Monoclonal Antibody for FC
Cat No. CL488-65085

产品说明书

CloneNo. 2H7

宿主/亚型

Mouse / Mouse IgG2b, kappa

种属反应性

Human

应用

FC

别名

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65085-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65085 targets CD20 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / Mouse IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
计算分子量 297 aa, 33 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
RRIDAB_2883167
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Affinity purification
UNIPROT IDP11836-1
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD20 antibody CL488-65085Download protocol
Standard Protocols
Click here to view our Standard Protocols