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CoraLite® Plus 405 Anti-Human CD20 (2H7)

CD20 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

2H7

Cat No : CL405-65085

Print datasheet

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

验证数据展示

  • FC experiment of human PBMCs using CL405-65085

    FC experiment of human PBMCs using CL405-65085

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL405-65085

    FC experiment of human PBMCs using CL405-65085

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65085-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65085 targets CD20 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen Human tonsillar B cells 种属同源性预测
Host / Isotype Mouse / Mouse IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 1
Synonyms B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC002807
Gene Symbol MS4A1
Gene ID (NCBI) 931
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD20 antibody CL405-65085Download protocol
Standard Protocols
Click here to view our Standard Protocols