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验证数据展示

  • WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

    WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01249-1

    Sandwich ELISA standard curve of MP01249-1

    Sandwich ELISA standard curve of MP01249-1, Mouse CD2 Recombinant Matched Antibody Pair - PBS only. 84360-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1403. 84360-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP01249-1

    Sample test of MP01249-1

    The mean CD2 concentration was determined to be 3.0 ng/mL in CTLL-2 cell extract based on a 0.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP01249-1

    Cytometric bead array standard curve of MP01249-1

    Cytometric bead array standard curve of MP01249-1, MOUSE CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84360-5-PBS. Detection antibody: 84360-4-PBS. Standard: Eg1403. Range: 0.313-40 ng/mL.

Mouse CD2 Recombinant antibody, PBS Only (Detector)

CD2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84360-4-PBS

产品说明书

CloneNo. 241548D7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84360-4-PBS targets CD2 as part of a matched antibody pair:

MP01249-1: 84360-5-PBS capture and 84360-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 antigen
别名 T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2
计算分子量38 kDa
观测分子量60 kDa
GenBank蛋白编号NM_013486.2
基因名称 CD2
Gene ID (NCBI) 12481
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08920
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.