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验证数据展示

  • WB analysis using 84360-4-RR

    WB analysis using 84360-4-RR

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse thymus using 84360-4-RR

    IHC staining of mouse thymus using 84360-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse thymus using 84360-4-RR

    IHC staining of mouse thymus using 84360-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD2 Recombinant antibody

CD2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84360-4-RR

产品说明书

CloneNo. 241548D7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCTLL-2 cells
Positive IHC detected inmouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84360-4-RR targets CD2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 antigen
别名 T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2
计算分子量38 kDa
观测分子量60 kDa
GenBank蛋白编号NM_013486.2
基因名称 CD2
Gene ID (NCBI) 12481
RRIDAB_3671898
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 84360-4-RRDownload protocol
IHC protocol for CD2 antibody 84360-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols