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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98192-1-RR

    Affinity and Kinetic Characterization of 98192-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98192-1-RR against Human CD16b were performed. The affinity constant is 2.36 nM.

Anti-Human CD16b Rabbit Recombinant Antibody, PBS Only

CD16b Uni-rAb® Recombinant Antibody for FC
Cat No. 98192-1-PBS

产品说明书

CloneNo. 241713E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

缓冲液配方:  PBS Only
HEPES, EDTA, CHAPS, Sucrose, Proclin 300
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98192-1-PBS targets CD16b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2487

Product name: recombinant human CD16b protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007018 of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
别名 CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta
基因名称 FCGR3B
Gene ID (NCBI) 2215
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).

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