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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR

    FC experiment of human peripheral blood leukocytes using 98192-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR

    FC experiment of human peripheral blood leukocytes using 98192-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

Anti-Human CD16b Rabbit Recombinant Antibody

CD16b Recombinant Antibody for FC
Cat No. 98192-1-RR

产品说明书

CloneNo. 241713E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

FCGR3B, FCG3, Fc gamma RIIIb, Fc gamma RIII beta, Fc gamma RIII

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
PE
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98192-1-RR targets CD16b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
别名 FCGR3B, FCG3, Fc gamma RIIIb, Fc gamma RIII beta, Fc gamma RIII
基因名称 FCGR3B
Gene ID (NCBI) 2215
RRIDAB_3672329
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).

实验方案

Product Specific Protocols
FC protocol for CD16b antibody 98192-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols