• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98293-1-RR

    FC experiment of human peripheral blood leukocytes using 98293-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98293-1-RR

    FC experiment of human peripheral blood leukocytes using 98293-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98293-1-RR

    Affinity and Kinetic Characterization of 98293-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a were performed. The affinity constant is 14.3 nM.

Anti-Human CD16a Rabbit Recombinant Antibody

CD16a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98293-1-RR

产品说明书

CloneNo. 242426G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98293-1-RR targets CD16a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.

实验方案

Product Specific Protocols
FC protocol for CD16a antibody 98293-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols