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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98293-1-RR

    Affinity and Kinetic Characterization of 98293-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a were performed. The affinity constant is 14.3 nM.

Anti-Human CD16a Rabbit Recombinant Antibody, PBS Only

CD16a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98293-1-PBS

产品说明书

CloneNo. 242426G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

98293-1-PBS targets CD16a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.