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CoraLite® Plus 488-conjugated CD163 Polyclonal antibody

CD163 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16646

Print datasheet

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

验证数据展示

  • IF Staining of human tonsillitis using CL488-16646

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-16646

    IF Staining of human tonsillitis using CL488-16646

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD163 antibody (CL488-16646) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman tonsillitis tissue, human liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 16646-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-16646 is CD163 Fusion Protein expressed in E. coli.

Tested Applications IF-P
Tested Reactivity human
Immunogen CD163 fusion protein Ag10029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD163 molecule
Synonyms CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_2919139
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VB7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD163 antibody CL488-16646Download protocol
Standard Protocols
Click here to view our Standard Protocols