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验证数据展示

  • WB analysis using 16646-1-AP

    WB analysis using 16646-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16646-1-AP (CD163 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16646-1-AP

    IHC staining of human placenta using 16646-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD163 antibody included
  • IHC staining of human placenta using 16646-1-AP

    IHC staining of human placenta using 16646-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD163 antibody included
  • IP experiment of human placenta using 16646-1-AP

    IP experiment of human placenta using 16646-1-AP

    IP result of anti-CD163 (IP:16646-1-AP, 4ug; Detection:16646-1-AP 1:2000 and HRP-conjugated Mouse Anti-Heavy Chain of Rabbit lgG SA00001-7H) with human placenta tissue lysate 1160 ug.

  • IF Staining of human tonsillitis using 16646-1-AP

    IF Staining of human tonsillitis using 16646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD163 antibody (16646-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 16646-1-AP

    IF Staining of human tonsillitis using 16646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD163 antibody (16646-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human lung cancer using 16646-1-AP

    IF Staining of human lung cancer using 16646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD163 antibody (16646-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 16646-1-AP

    IF Staining of human placenta using 16646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD163 antibody (16646-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CD163 Polyclonal antibody

CD163 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 16646-1-AP

产品说明书

引用文献(339)

宿主/亚型

Rabbit / IgG

种属反应性

human, pig and More (3)

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, pig spleen tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human liver tissue, human lung tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human liver tissue, human placenta tissue, human lung cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16646-1-AP targets CD163 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, pig
文献引用反应性human, mouse, rat, pig, monkey
免疫原

CatNo: Ag10029

Product name: Recombinant human CD163 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 46-401 aa of BC051281

Sequence: GTDKELRLVDGENKCSGRVEVKVQEEWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKHSNCTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVSFSGSSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECSGRLEVRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAVWQCKHHEWGKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD163 molecule
别名 CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130
计算分子量 1156 aa, 125 kDa
观测分子量 130-150 kDa
GenBank蛋白编号BC051281
基因名称 CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_2756528
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

实验方案

Product Specific Protocols
IF protocol for CD163 antibody 16646-1-APDownload protocol
IHC protocol for CD163 antibody 16646-1-APDownload protocol
IP protocol for CD163 antibody 16646-1-APDownload protocol
WB protocol for CD163 antibody 16646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Cancer Cell

Tissue tension fosters macrophage-driven lipid peroxidation-induced DNA damage.

Authors - Mary-Kate Hayward
View Article
mouse

Nat Med

Concurrent intrathecal and intravenous nivolumab in leptomeningeal disease: phase 1 trial interim results

Authors - Isabella C Glitza Oliva
View Article
mouseIF

Cell

Extracellular vesicles from the lung pro-thrombotic niche drive cancer-associated thrombosis and metastasis via integrin beta 2

Authors - Serena Lucotti
View Article
mouseIF

Gastroenterology

Targeting Stem Cells and Dysplastic Features With Dual MEK/ERK and STAT3 Suppression in Gastric Carcinogenesis

Authors - Hyesung Kim
View Article
mouseIF

Gastroenterology

Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis

Authors - Tao Zuo
View Article
mouseIF

Adv Mater

Endogenous Metal Ion-Enriched Immunostimulating Biomimetic Scaffold Improves In Situ Bone Regeneration.

Authors - Min Yu
View Article
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