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CoraLite® Plus 488 Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC, IF/ICC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse

Applications

FC, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

93

Cat No : CL488-65057

Print datasheet

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

验证数据展示

  • FC experiment of BALB/c mouse splenocytes using CL488-65057

    FC experiment of BALB/c mouse splenocytes using CL488-65057

    1X10^6 BALB/c mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD16/32 (CL488-65057, Clone:93) or 0.5 ug Isotype Control. Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65057

    IF Staining of mouse splenocytes using CL488-65057

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD16/32 antibody (CL488-65057, Clone: 93 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65057-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65057 targets CD16/32 in IF, FC applications and shows reactivity with Mouse samples.

Tested Applications FC, IF/ICC
Tested Reactivity Mouse
Immunogen VH81X Tg B cells and T220 stromal cell line 种属同源性预测
Host / Isotype Rat / IgG2a, lambda
Class Monoclonal
Type Antibody
Full Name Fc receptor, IgG, low affinity IIb
Synonyms AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1
GenBank Accession NumberBC038070
Gene Symbol Fcgr2b
Gene ID (NCBI) 14130
RRIDAB_2919253
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08101
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD16/32 antibody CL488-65057Download protocol
Standard Protocols
Click here to view our Standard Protocols