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验证数据展示

  • FC experiment of human PBMCs using 65568-1-MR (same clone as 65568-1-PBS)

    FC experiment of human PBMCs using 65568-1-MR (same clone as 65568-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD11c (3.9) Mouse Recombinant Antibody (65568-1-MR, Clone:3.9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were not fixed. This data was developed using the same antibody clone with 65568-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65568-1-PBS

    FC experiment of human PBMCs using 65568-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11c (3.9) Mouse IgG2a RecAb, PBS Only (65568-1-PBS, Clone: 3.9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 65568-1-PBS

    FC experiment of human PBMCs using 65568-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11c (3.9) Mouse IgG2a RecAb, PBS Only (65568-1-PBS, Clone: 3.9) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD11c (3.9) Mouse IgG2a Recombinant Antibody, PBS Only

CD11c Recombinant Antibody for FC
Cat No. 65568-1-PBS

产品说明书

CloneNo. 3.9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

3.9, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs, FC receptor blocked human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65568-1-PBS targets CD11c in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha X (complement component 3 receptor 4 subunit)
别名 3.9, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX
计算分子量 1169 aa, 129 kDa
GenBank蛋白编号BC038237
基因名称 CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20702
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CD11c antibody 65568-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols