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验证数据展示

  • IF Staining of NIH/3T3 using APC-65052

    IF Staining of NIH/3T3 using APC-65052

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using APC-65052 (CD107b antibody) at dilution of 1:100.

  • FC experiment of NIH/3T3 using APC-65052

    FC experiment of NIH/3T3 using APC-65052

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug APC Anti-Mouse CD107b / LAMP2 (APC-65052, Clone:ABL-93) or 0.25 ug APC Rat IgG2a Isotype Control (2A3) (APC-65209, Clone: 2A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

APC Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. APC-65052

产品说明书

CloneNo. ABL-93

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

IF/ICC, FC (Intra)

别名

ABL93, CD107b, Lamp2, Mac3, Lamp 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格价格库存

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65052 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 mouse
免疫原 Glycoproteins purified from BALB/c mouse embryo 3T3 cell line 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 ABL93, CD107b, Lamp2, Mac3, Lamp 2
GenBank蛋白编号BC138718
基因名称 CD107b / LAMP2
Gene ID (NCBI) 16784
RRIDAB_2882956
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP17047
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
IF protocol for APC CD107b / LAMP2 antibody APC-65052Download protocol
FC protocol for APC CD107b / LAMP2 antibody APC-65052Download protocol
Standard Protocols
Click here to view our Standard Protocols