验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse ovary tissue, HepG2 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12628-1-AP targets CCT6B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3321 Product name: Recombinant human CCT6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 231-530 aa of BC027591 Sequence: LICNVSLEYEKTEVNSGFFYKTAEEKEKLVKAERKFIEDRVQKIIDLKDKVCAQSNKGFVVINQKGIDPFSLDSLAKHGIVALRRAKRRNMERLSLACGGMAVNSFEDLTVDCLGHAGLVYEYTLGEEKFTFIEECVNPCSVTLLVKGPNKHTLTQVKDAIRDGLRAIKNAIEDGCMVPGAGAIEVAMAEALVTYKNSIKGRARLGVQAFADALLIIPKVLAQNAGYDPQETLVKVQAEHVESKQLVGVDLNTGEPMVAADAGVWDNYCVKKQLLHSCTVIATNILLVDEIMRAGMSSLK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chaperonin containing TCP1, subunit 6B (zeta 2) |
| 别名 | CCT zeta 2, CCTZ 2, Cctz2, CCT-zeta-2, CCT-zeta-like |
| 计算分子量 | 530 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC027591 |
| 基因名称 | CCT6B |
| Gene ID (NCBI) | 10693 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92526 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, testis-specific Tcp20 and testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role in vitro, in the folding of actin and tubulin. This antibody can recognize both of CCT6A and CCT6B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCT6B antibody 12628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
