验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98501-2-RR targets CCL22/MDC in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2241 Product name: Recombinant Mouse CCL22 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 25-92 aa of NM_009137 Sequence: GPYGANVEDSICCQDYIRHPLPSRLVKEFFWTSKSCRKPGVVLITVKNRDICADPRQVWVKKLLHKLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 22 |
| 别名 | Abcd1, Activated B and dendritic cell-derived, CC chemokine ABCD-1, C-C motif chemokine 22, Ccl22 |
| 计算分子量 | 10kd |
| GenBank蛋白编号 | NM_009137 |
| 基因名称 | CCL22/MDC |
| Gene ID (NCBI) | 20299 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O88430 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CCL22/MDC antibody 98501-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
