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验证数据展示

  • FC experiment of Transfected HEK-293T using 98464-1-RR

    FC experiment of Transfected HEK-293T using 98464-1-RR

    1x10^6 HEK-293T cells (left) CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98464-1-RR

    FC experiment of Transfected HEK-293T using 98464-1-RR

    1x10^6 HEK-293T cells (left) or CCL11/Eotaxin-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL11/Eotaxin Rabbit RecAb (98464-1-RR, Clone: 243012F8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Mouse CCL11/Eotaxin Rabbit Recombinant Antibody

CCL11/Eotaxin Recombinant Antibody for FC (Intra)
Cat No. 98464-1-RR

产品说明书

CloneNo. 243012F8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

C-C motif chemokine 11, Ccl11, Eosinophil chemotactic protein, Eotaxin, Scya11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98464-1-RR targets CCL11/Eotaxin in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 CCL11/Eotaxin fusion protein Eg2400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 11
别名 C-C motif chemokine 11, Ccl11, Eosinophil chemotactic protein, Eotaxin, Scya11
计算分子量11kd
GenBank蛋白编号NM_011330
基因名称 Ccl11
Gene ID (NCBI) 20292
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48298
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CCL11 also named Eotaxin-1, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.

实验方案

Product Specific Protocols
FC protocol for CCL11/Eotaxin antibody 98464-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols