• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83258-6-RR (same clone as 83258-6-PBS)

    WB analysis using 83258-6-RR (same clone as 83258-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83258-6-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 83258-6-RR (same clone as 83258-6-PBS)

    WB analysis of K-562 using 83258-6-RR (same clone as 83258-6-PBS)

    WB result of CBX5 antibody (83258-6-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells. This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83258-6-RR

    Affinity and Kinetic Characterization of 83258-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83258-6-RR against Human CBX5 were performed. The affinity constant is 0.606 nM.

  • Featured Product
  • KD/KO验证

CBX5 Recombinant antibody, PBS Only

CBX5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83258-6-PBS

产品说明书

CloneNo. 240145G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

240145G12, Antigen p25, HP1, HP1 alpha, HP1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CBX5 Ready-To-Use IHC Kit. CBX5 primary antibody included.

产品信息

83258-6-PBS targets CBX5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CBX5 fusion protein Ag34810 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromobox homolog 5 (HP1 alpha homolog, Drosophila)
别名 240145G12, Antigen p25, HP1, HP1 alpha, HP1A
计算分子量 191 aa, 22 kDa
观测分子量24 kDa
GenBank蛋白编号BC006821
基因名称 CBX5
Gene ID (NCBI) 23468
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP45973
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443).