• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CBS Monoclonal antibody, PBS Only

CBS Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E4F10

Cat No : 67861-1-PBS

Print datasheet

Synonyms

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase

验证数据展示

  • IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • WB analysis using 67861-1-Ig (same clone as 67861-1-PBS)

    WB analysis using 67861-1-Ig (same clone as 67861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

产品信息

67861-1-PBS targets CBS in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, rat
Immunogen CBS fusion protein Ag7008 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cystathionine-beta-synthase
Synonyms Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61-63 kDa
GenBank Accession NumberBC000440
Gene Symbol CBS
Gene ID (NCBI) 875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35520
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).