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验证数据展示

  • IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • WB analysis using 67861-1-Ig (same clone as 67861-1-PBS)

    WB analysis using 67861-1-Ig (same clone as 67861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • WB analysis of NCI-H1299 using 67861-1-Ig (same clone as 67861-1-PBS)

    WB analysis of NCI-H1299 using 67861-1-Ig (same clone as 67861-1-PBS)

    WB result of CBS antibody (67861-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells. This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    IHC staining of human pancreas cancer using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    IF Staining of HeLa using 67861-1-Ig (same clone as 67861-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67861-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CBS Monoclonal antibody, PBS Only

CBS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67861-1-PBS

产品说明书

CloneNo. 1E4F10

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase, Beta thionase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67861-1-PBS targets CBS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag7008

Product name: Recombinant human CBS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 206-551 aa of BC000440

Sequence: VAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cystathionine-beta-synthase
别名 EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase, Beta thionase
计算分子量 61 kDa
观测分子量 61-63 kDa
GenBank蛋白编号BC000440
基因名称 CBS
Gene ID (NCBI) 875
RRIDAB_2918619
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35520
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

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