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验证数据展示

  • WB analysis using 83300-3-RR

    WB analysis using 83300-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83300-3-RR

    WB analysis of HEK-293 using 83300-3-RR

    WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells.

  • FC experiment of HepG2 using 83300-3-RR

    FC experiment of HepG2 using 83300-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CARHSP1 Recombinant antibody

CARHSP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83300-3-RR

产品说明书

CloneNo. 230489A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, HEK-293T cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83300-3-RR targets CARHSP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CARHSP1 fusion protein Ag2265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium regulated heat stable protein 1, 24kDa
别名 CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1
计算分子量 147 aa, 16 kDa
观测分子量22 kDa
GenBank蛋白编号BC003366
基因名称 CARHSP1
Gene ID (NCBI) 23589
RRIDAB_3670970
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2V2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

实验方案

Product Specific Protocols
WB protocol for CARHSP1 antibody 83300-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols