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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83300-3-RR (same clone as 83300-3-PBS)

    WB analysis using 83300-3-RR (same clone as 83300-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83300-3-RR (same clone as 83300-3-PBS)

    WB analysis of HEK-293 using 83300-3-RR (same clone as 83300-3-PBS)

    WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83300-3-RR (same clone as 83300-3-PBS)

    FC experiment of HepG2 using 83300-3-RR (same clone as 83300-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83300-3-PBS in a different storage buffer formulation.

CARHSP1 Recombinant antibody, PBS Only

CARHSP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83300-3-PBS

产品说明书

CloneNo. 230489A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83300-3-PBS targets CARHSP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CARHSP1 fusion protein Ag2265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium regulated heat stable protein 1, 24kDa
别名 CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1
计算分子量 147 aa, 16 kDa
观测分子量22 kDa
GenBank蛋白编号BC003366
基因名称 CARHSP1
Gene ID (NCBI) 23589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2V2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).