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验证数据展示

  • IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

    IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

    IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 66352-1-Ig (same clone as 66352-1-PBS)

    FC experiment of NIH/3T3 using 66352-1-Ig (same clone as 66352-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • WB analysis using 66352-1-Ig (same clone as 66352-1-PBS)

    WB analysis using 66352-1-Ig (same clone as 66352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51429-1

    Cytometric bead array sample test of MP51429-1

    Cytometric bead array sample test of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS.

  • Cytometric bead array standard curve of MP51429-1

    Cytometric bead array standard curve of MP51429-1

    Cytometric bead array standard curve of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS. Standard:Ag7413. Range: 1.563-200 ng/mL.

CAPRIN1 Monoclonal antibody, PBS Only (Capture)

CAPRIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66352-1-PBS

产品说明书

CloneNo. 6B10F11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

6B10F11, caprin 1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.

产品信息

66352-1-PBS targets CAPRIN1 as part of a matched antibody pair:

MP51429-1: 66352-1-PBS capture and 66352-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7413

Product name: Recombinant human CAPRIN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 356-709 aa of BC001731

Sequence: RQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cell cycle associated protein 1
别名 6B10F11, caprin 1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1
计算分子量 78 kDa
观测分子量 116 kDa
GenBank蛋白编号BC001731
基因名称 CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2881732
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14444
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

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