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CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

6B10F11

Cat No : 66352-1-Ig

Print datasheet

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105

验证数据展示

  • IHC staining of human breast cancer using 66352-1-Ig

    IHC staining of human breast cancer using 66352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPRIN1 antibody included
  • IF Staining of HeLa using 66352-1-Ig

    IF Staining of HeLa using 66352-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 66352-1-Ig

    WB analysis using 66352-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of NIH/3T3 using 66352-1-Ig

    FC experiment of NIH/3T3 using 66352-1-Ig

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected insodium arsenite treated HeLa cells, HeLa cells
Positive FC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66352-1-Ig targets CAPRIN1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen CAPRIN1 fusion protein Ag7413 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cell cycle associated protein 1
Synonyms caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2881732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
WB protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
IHC protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
IF protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols