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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CAPG Monoclonal antibody

CAPG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A5B2

Cat No : CL488-66277

Print datasheet

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

验证数据展示

  • IF Staining of HeLa using CL488-66277

    IF Staining of HeLa using CL488-66277

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66277 (CAPG antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66277-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66277 targets CAPG in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen CAPG fusion protein Ag24676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament), gelsolin-like
Synonyms Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC000728
Gene Symbol CAPG
Gene ID (NCBI) 822
RRIDAB_2883300
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40121
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAPG antibody CL488-66277Download protocol
Standard Protocols
Click here to view our Standard Protocols