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验证数据展示

  • WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

    IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

    IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of mouse lung using 68207-1-Ig (same clone as 68207-1-PBS)

    IHC staining of mouse lung using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CAP1 Monoclonal antibody, PBS Only

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68207-1-PBS

产品说明书

CloneNo. 2H3A10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.

产品信息

68207-1-PBS targets CAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag8876

Product name: Recombinant human CAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-475 aa of BC013963

Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CAP, adenylate cyclase-associated protein 1 (yeast)
别名 2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN
计算分子量 475 aa, 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC013963
基因名称 CAP1
Gene ID (NCBI) 10487
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ01518
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

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