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验证数据展示

  • WB analysis using 68207-1-Ig

    WB analysis using 68207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68207-1-Ig

    IF Staining of HepG2 using 68207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 68207-1-Ig

    IF Staining of HepG2 using 68207-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HepG2 using 68207-1-Ig

    WB analysis of HepG2 using 68207-1-Ig

    WB result of CAP1 antibody (68207-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAP1 transfected HepG2 cells.

  • WB analysis of pig lung using 68207-1-Ig

    WB analysis of pig lung using 68207-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 68207-1-Ig

    WB analysis of human placenta using 68207-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 68207-1-Ig

    IHC staining of human colon cancer using 68207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of mouse testis using 68207-1-Ig

    IHC staining of mouse testis using 68207-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of mouse lung using 68207-1-Ig

    IHC staining of mouse lung using 68207-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAP1 antibody included
  • IF Staining of HeLa using 68207-1-Ig

    IF Staining of HeLa using 68207-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

CAP1 Monoclonal antibody

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68207-1-Ig

产品说明书

CloneNo. 2H3A10
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, human placenta tissue, pig lung tissue, mouse lung tissue, rat lung tissue, rabbit lung tissue
Positive IHC detected inhuman colon cancer tissue, mouse lung tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68207-1-Ig targets CAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原

CatNo: Ag8876

Product name: Recombinant human CAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-475 aa of BC013963

Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CAP, adenylate cyclase-associated protein 1 (yeast)
别名 2H3A10, Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN
计算分子量 475 aa, 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC013963
基因名称 CAP1
Gene ID (NCBI) 10487
RRIDAB_2935295
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ01518
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

实验方案

Product Specific Protocols
IF protocol for CAP1 antibody 68207-1-IgDownload protocol
IHC protocol for CAP1 antibody 68207-1-IgDownload protocol
WB protocol for CAP1 antibody 68207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Int Immunopharmacol

Single-cell RNA sequencing reveals cell-cell communication and potential biomarker in sepsis and septic shock patients

Authors - Guilin Li
View Article
humanIF

Acta Neuropathol

Enhanced microglial dynamics and a paucity of tau seeding in the amyloid plaque microenvironment contribute to cognitive resilience in Alzheimer's disease

Authors - Nur Jury-Garfe
View Article
humanWB

J Neurochem

CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation

Authors - Jie Yang
View Article
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