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验证数据展示

  • WB analysis using 16231-1-AP (same clone as 16231-1-PBS)

    WB analysis using 16231-1-AP (same clone as 16231-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 16231-1-AP (same clone as 16231-1-PBS)

    IP experiment of A431 using 16231-1-AP (same clone as 16231-1-PBS)

    IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

    IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

    IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • Featured Product
  • KD/KO Validated

CAP1 Polyclonal antibody, PBS Only

CAP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16231-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.

产品信息

16231-1-PBS targets CAP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8876

Product name: Recombinant human CAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-475 aa of BC013963

Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CAP, adenylate cyclase-associated protein 1 (yeast)
别名 Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN
计算分子量 475 aa, 52 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC013963
基因名称 CAP1
Gene ID (NCBI) 10487
RRIDAB_2172166
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01518
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

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