验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, COLO 320 cells, HepG2 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16231-1-AP targets CAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 | CAP1 fusion protein Ag8876 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CAP, adenylate cyclase-associated protein 1 (yeast) |
| 别名 | Adenylyl cyclase associated protein 1, CAP, CAP 1, CAP1, CAP1 PEN |
| 计算分子量 | 475 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC013963 |
| 基因名称 | CAP1 |
| Gene ID (NCBI) | 10487 |
| RRID | AB_2172166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01518 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAP1 antibody 16231-1-AP | Download protocol |
| IHC protocol for CAP1 antibody 16231-1-AP | Download protocol |
| IP protocol for CAP1 antibody 16231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.
| ||
Viruses Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage. | ||
Cell Biochem Funct Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.
| ||
Cell Mol Life Sci Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology |
