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验证数据展示

  • WB analysis using CL488-66843

    WB analysis using CL488-66843

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66843 (CAMK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated CAMK2 Monoclonal antibody

CAMK2 Monoclonal Antibody for WB
Cat No. CL488-66843

产品说明书

CloneNo. 1E3B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB

别名

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy® CAMK2A Ready-To-Use IHC Kit. CAMK2A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66843-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66843 targets CAMK2 in WB applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag4736

Product name: Recombinant human CAMK2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-271 aa of BC040457

Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II alpha
别名 CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha
计算分子量 478 aa, 54 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC040457
基因名称 CaMKII alpha
Gene ID (NCBI) 815
RRIDAB_3084277
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UQM7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CAMK2 antibody CL488-66843Download protocol
Standard Protocols
Click here to view our Standard Protocols
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